FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62451341
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC166000.010.5740361
CTCACGC516850.09.448293
GCTCACG535550.09.1885472
CGCGATC111950.08.7500863
GCGCGAT113400.08.7044842
ACCGCAC174600.07.7912351
GGCGCGA138250.07.45918851
CCGCGCC251200.07.39669132
TCACGCC692550.07.298074
CACGCCT715750.07.17031675
AACGCGA29201.0913936E-117.01314451
ACGCCTG692550.06.92904336
CGCCTGT750500.06.79001957
CGCGCCC204250.06.6077493
GCGATCT234450.06.55710274
ACCGTGC198700.06.55178641
ACACGTC544500.06.4055204145
CGTCTGA485700.06.3449483145
GATCGGA836850.05.9527326145
CCGCACC200250.05.7991542
Produced by FastQC (version 0.11.5)