FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62451341
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG63100.012.299416145
ACCGCGC176900.010.9829611
CTCACGC522350.08.7776533
GCTCACG531750.08.4619492
CGCGATC106650.08.3669513
GCGCGAT108200.08.1831172
ACCGCAC179250.07.21239851
CCGCGCC295000.06.79005532
TCACGCC683000.06.73409134
CACGCCT700550.06.663835
ACGCCTG674500.06.6632146
CGCCTGT744900.06.5297817
ACCGTGC199600.06.4770671
GGCGCGA142350.06.46515131
CGTCGTG535150.06.465065145
GCGATCT218650.06.43669134
AGCGTCG592900.06.312455145
TCGTGTA516350.05.9981008145
CGCGCCC252550.05.9175623
GGCTCAC1668750.05.7250871
Produced by FastQC (version 0.11.5)