FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64719989
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG57750.010.427967145
ACCGCGC186950.09.6499861
CTCACGC562100.08.0875833
GCTCACG567050.07.86808822
CGCGATC112850.07.7740713
GCGCGAT112800.07.13886832
CACGCCT745850.06.5027355
ACGCCTG712800.06.43809656
TCACGCC726850.06.36415344
CGTCGTG586850.06.2807307145
GGCGCGA143200.06.27360731
CGCCTGT783750.06.1512817
CCGCGCC310000.06.0143252
GCGATCT232250.05.83781964
AGCGTCG640800.05.785914145
CGTGTAG577700.05.764795145
ACCGTGC206950.05.71748161
ACCGCAC192000.05.7061771
GTCGTGT593300.05.686593145
GCGTCGT597250.05.6272597140-144
Produced by FastQC (version 0.11.5)