FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64999330
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT114500.010.645142
CGCGATC117100.010.5300383
ACCGCGC187200.010.0140471
GCGTCGG59300.09.906122145
GGCGCGA148100.08.5206621
GCTCACG564550.08.0834322
CTCACGC556700.07.9608213
GCGATCT241900.07.76592874
CGTCGTG600350.06.523229145
TCACGCC722000.06.3088764
ACCGCAC188850.06.2572041
ACGCCTG717200.06.2157286
ACCGTGC213600.06.21516131
CACGCCT744700.06.20033555
CGCCTGT776600.06.11358177
AGCGTCG659050.06.008245145
CGTGTAG593850.05.935166145
GATCGGA1011650.05.8353577145
GTCGTGT608200.05.652041145
GAGCGTC743800.05.635663145
Produced by FastQC (version 0.11.5)