FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62084643
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG53150.015.009414145
CGTCGTG666300.06.465334145
ACCGCGC170150.06.387851
CGTGTAG650450.06.355288145
AGCGTCG738400.06.1777916145
GAGCGTC822100.06.1575093145
TCGTGTA642300.05.9955754145
GTCGTGT668550.05.825252145
GCGTCGT687950.05.776734140-144
TCGGAAG1095700.05.7186875145
ATCGGAA1114400.05.6747885145
AGATCGG1177750.05.6528034145
CGCGATC102100.05.6122443
CGGAAGA1083200.05.46331145
GTCGCCG181700.05.428231145
GATCGGA1126550.05.4268947145
TAGATCG433950.05.3646283145
GCGCGAT103050.05.35071852
AAGAGCG1000650.05.2472367145
CGCCGTA137350.05.227341145