FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61892582
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG55500.011.366218145
ACCGCGC166900.06.7334631
CGTGTAG625400.06.573776145
TCGTGTA621300.06.3487353145
CGTCGTG647000.06.163794145
AGCGTCG707300.06.1508803145
GCGTCGT663950.05.985959140-144
TCGCCGT145750.05.920087145
GTCGTGT645850.05.8267365145
GATCGGA1076600.05.65737145
AGATCGG1131450.05.613819145
CGCGATC103400.05.60899973
GCGCGAT102650.05.50674252
ATCGGAA1064550.05.4898276145
CGCCGTA125750.05.477789145
GAGCGTC789500.05.446183145
AGAGCGT861800.05.351066145
AAGAGCG964150.05.324494145
CGGAAGA1047850.05.3074503145
TCGGAAG1052800.05.2962704145
Produced by FastQC (version 0.11.5)