FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82043644
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA444550.029.1494391
TCGGAAG505050.025.8567732
CGGAAGA542200.024.1209243
GATCGGA329400.017.4077761
TATGCCG274850.013.76343345-49
CTCGTAT266250.013.66879140-44
AGAGCAC1022250.013.3325067
TCTCGTA280950.013.08778740-44
AGCACAC1077500.012.790239
CGTATGC300850.012.62698745-49
GAAGAGC1111650.012.15595155
GAGCACA1132750.012.1407178
ATGCCGT333000.011.5080445-49
ATCTCGT336650.011.07310740-44
GCCGTCT391300.09.83409850-54
AAGAGCA1459850.09.5842946
CGTCTTC418600.09.29665650-54
CCGTCTT431750.09.00678650-54
TCGTATG288250.08.94351940-44
ACACGTC453150.08.49505110-14
Produced by FastQC (version 0.11.5)