FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82043644
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT823930.10042581726379682Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG589000.022.1190819
ATCGGAA696000.019.0168821
GAGCGTC696000.018.8644088
AGAGCGT712150.018.4162457
TCGGAAG776900.017.1436562
CGGAAGA806750.016.3832953
AAGAGCG853950.015.4770526
GAAGAGC1371300.010.0292175
GATCGGA591200.09.7280211
CGCCGTA414450.09.11390145-49
GCCGTAT434000.08.80024445-49
CGTATCA449500.08.53872445-49
CCGTATC464500.08.27547145-49
TGGTCGC525950.07.372091340-44
GTCGCCG531050.07.32313640-44
ACCGCGC386450.07.2815541
TCGTGTA533400.07.119531610-14
GCGTCGT545250.06.924911510-14
GTGGTCG567000.06.866492740-44
CGTCGTG556050.06.79041110-14
Produced by FastQC (version 0.11.5)