FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82701090
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA410800.026.9220771
TCGGAAG474250.023.4541322
CGGAAGA514900.021.7008973
GATCGGA312850.014.7943031
TATGCCG246700.013.000339545-49
CTCGTAT237750.012.93450940-44
TCTCGTA254000.012.11842340-44
AGAGCAC992800.011.84328657
CGTATGC271500.011.72204745-49
AGCACAC1047900.011.1444569
GAAGAGC1092350.010.8102935
GAGCACA1107100.010.6860338
ATGCCGT310500.010.427155545-49
ATCTCGT310900.010.1896840-44
GCCGTCT366550.08.97858250-54
CGTCTTC396800.08.370831550-54
AAGAGCA1441000.08.3708116
CCGTCTT408250.08.16446950-54
TCGTATG266500.08.16063440-44
ACACGTC424800.07.70298810-14
Produced by FastQC (version 0.11.5)