FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82701090
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG550700.020.142069
GAGCGTC656100.017.138368
ATCGGAA660650.016.6776491
AGAGCGT671150.016.4191827
TCGGAAG735400.014.9672492
CGGAAGA769000.014.3233473
AAGAGCG813850.013.6204236
GAAGAGC1354550.08.5528185
CGCCGTA379600.08.41484245-49
GCCGTAT396500.08.18051145-49
GATCGGA571100.08.1393261
CGTATCA422400.07.62055745-49
CCGTATC432450.07.540692345-49
ACCGCGC388050.06.62576871
TGGTCGC493750.06.59569240-44
GTCGCCG501500.06.54291640-44
TCGTGTA499550.06.455254610-14
GCGTCGT505300.06.281364410-14
GTGGTCG533650.06.17318840-44
CGTCGTG517550.06.16630910-14
Produced by FastQC (version 0.11.5)