FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82661578
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG566800.020.1198029
GAGCGTC681100.016.9243148
ATCGGAA685500.016.7593361
AGAGCGT694600.016.6058187
TCGGAAG756350.015.2307672
CGGAAGA792650.014.6020793
AAGAGCG833050.013.88086
GATCGGA587900.09.6286731
GAAGAGC1363350.08.8485245
CGCCGTA406550.08.47754445-49
GCCGTAT420650.08.30023645-49
CGTATCA433450.08.08188745-49
CCGTATC445950.07.913876545-49
TGGTCGC513650.06.966789240-44
GTCGCCG524950.06.71738840-44
TCGTGTA519150.06.6723410-14
GCGTCGT524500.06.601516210-14
GTGGTCG552500.06.511024540-44
CGTCGTG536900.06.435547410-14
ACCGCGC392650.06.4032861
Produced by FastQC (version 0.11.5)