FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60706989
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC167450.013.5203181
CGCGATC100000.012.25336553
GCGCGAT99650.011.7146532
CTCACGC510400.011.4922893
GCTCACG516800.011.4344682
ACCGCAC178300.09.8942141
GGCGCGA124000.09.7810081
CACGCCT679650.09.3654245
ACGCCTG657950.09.2443326
TCACGCC659500.09.0150414
CGCCTGT710800.08.9955197
CCTTCGT216650.08.9910121
GCGATCT216450.08.9438064
CCGCGCC264400.08.5560642
ACCGTGC190350.08.49554351
CGCGCCC216550.07.8682543
ACTGCGC174700.07.78395271
CTTCGTT250000.07.6567552
TCGTTGG320750.07.55002744
ACTGCAC1302900.07.49218271
Produced by FastQC (version 0.11.5)