FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005753981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005753981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61919376
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC162800.013.8821691
GCTCACG531550.012.1511992
CTCACGC524350.012.0692883
GCGCGAT111750.011.8194472
CGCGATC112100.011.76940253
ACCGCAC163600.010.4952691
GGCGCGA135000.010.1640971
CCTTCGT223100.09.5380461
ACCGTGC192650.09.5220831
CACGCCT705150.09.365335
ACGCCTG678650.09.1969436
TCACGCC692250.09.1105074
CCGCGCC248450.09.0551572
CGCCTGT739200.08.8750927
GCGATCT229100.08.8280614
ACTGCGC185250.08.6349391
CTTCGTT251450.08.5430572
TCGTTGG328600.08.4712964
CGCGCCC202600.08.1937883
CGCAATC107350.08.1709393
Produced by FastQC (version 0.11.5)