FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407642635
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2033850.016.9713769
GAGCGTC2415150.014.4210438
ATCGGAA2797900.013.0866621
AGAGCGT2737950.012.8823517
TCGGAAG2942600.012.4594682
CGGAAGA3212150.011.4410083
AAGAGCG3332050.010.9270526
CGCCGTA942050.06.79400955-59
GAAGAGC6018000.06.44524435
ACCGCGC1074900.06.2191721
TCGCCGT1045150.06.12103255-59
GCCGTAT1132150.05.82612455-59
GTCGCCG1239650.05.82151955-59
CGCGATC552050.05.34507473
CCGTATC1235600.05.341855555-59
TGGTCGC1379400.05.297952750-54
CGTATCA1318700.05.162467560-64
CCTTCGT1569200.05.14726541
CTCACGC3257950.05.12269743
GGTCGCC1424900.05.118602350-54
Produced by FastQC (version 0.11.5)