FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360712404
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1643350.022.8747569
GAGCGTC1955950.019.2485688
ATCGGAA2275100.017.2888531
AGAGCGT2237400.016.869357
TCGGAAG2405450.016.157992
CGGAAGA2614750.014.9062013
AAGAGCG2710950.014.2381756
CGCCGTA734650.09.07717555-59
TCGCCGT818250.08.19229755-59
GAAGAGC5071550.08.1055065
GTCGCCG981800.07.85108655-59
GCCGTAT926100.07.44492755-59
TGGTCGC1097400.07.05312250-54
GGTCGCC1134050.06.88271850-54
CCGTATC1018150.06.794624355-59
CGTATCA1110400.06.41556860-64
GTGGTCG1249600.06.353029750-54
TCTTCGC1467300.05.54187730-34
GTCTTCG1503900.05.426289630-34
TCTCGGT1557050.05.39006245-49
Produced by FastQC (version 0.11.5)