FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461721497
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG270200.012.8621220-24
AGCGTCG1406100.012.8374079
TGGCCCG1087850.012.4742345
GAGCGTC1741150.010.5253538
ATCGGAA2018200.09.19846251
AGAGCGT2164400.08.5973837
TCGGAAG2204000.08.4951492
ATCGCGG421350.08.44081620-24
GTATGGC1897950.07.7338972
GGCCCGG1847600.07.54481176
CGGAAGA2533700.07.47286033
GATCCGT470750.07.17571931
TCGCGGC516450.07.08583320-24
CCGGGGG2031800.06.74333959
AAGAGCG2784050.06.73851736
GCCCGGG2102200.06.7207187
CCCGGGG2161450.06.5401128
TATGGCC2580550.05.90458443
GGATCCG527100.05.899834
ATGGCCC2706950.05.6557334
Produced by FastQC (version 0.11.5)