FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409693288
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG223350.013.216372520-24
TGGCCCG910750.012.0666775
ATCGCGG298400.010.06717520-24
CCGGGGG1284150.08.608759
GGCCCGG1375300.08.3437076
ATCGGAA1009000.08.1923051
TCGCGGC393500.07.928781520-24
TCGGAAG1112050.07.52417662
CCCGGGG1577050.07.2349648
GCCCGGG1590150.07.21636347
GTATGGC1719000.06.8103082
CGGAAGA1345900.06.3298853
GATCCGT409750.06.31191061
GGATCCG437600.05.4961944
TATGGCC2343600.05.44039543
TTCGCGA142000.05.348934730-34
ATGGCCC2390650.05.193934
GTACTGG2429700.05.1344281
Produced by FastQC (version 0.11.5)