FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399601484
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG220550.010.79429320-24
TGGCCCG904750.09.8073085
ACCGCGC985750.09.5534681
GGATCCG446550.08.0033874
CGCGATC490200.07.6752243
GCGCGAT488850.07.3998332
ATCGCGG351350.07.03165720-24
CTCACGC3031250.07.0239143
CCTTCGT1471650.06.90163951
GCTCACG3071500.06.88938672
ACCGTGC1155650.06.84411241
CCGCGCC1533750.06.2673792
GTATGGC1622400.06.2064222
GGCCCGG1499550.06.0670846
ACCGCAC1026900.06.0502231
TCGCGGC421600.06.011307220-24
GATCCGT434750.06.00318341
CTTCGTT1716850.05.873432
TCACGCC3765850.05.8597444
TCGTTGG2084950.05.76482154
Produced by FastQC (version 0.11.5)