FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390489913
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1156400.031.8128491
TCGGAAG1273650.028.7195822
CGGAAGA1503350.024.3699343
GGATCCG426150.011.3514734
TATGCCG707850.010.60220745-49
AGAGCAC3723500.010.3856297
TATCGCG227350.09.81735120-24
CGGCTAT779650.09.81176830-34
AGCACAC3985450.09.7792189
CTCGTAT781300.09.48043640-44
GAGCACA4154950.09.4185658
GAAGAGC4216050.09.3283745
TGGCCCG829450.09.1187255
CGTATGC827350.08.9710645-49
ACCGGCT950200.08.16948630-34
TCTCGTA701200.07.85595840-44
TGCCGTC880600.07.79034945-49
TCACCGG987600.07.74392125-29
ATGCCGT919950.07.734246745-49
ATCGCGG299850.07.56906520-24
Produced by FastQC (version 0.11.5)