FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400343319
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1257000.040.957511
TCGGAAG1370350.037.5959172
CGGAAGA1611400.031.9764163
ACCGCGC1048300.018.049411
AGAGCAC3876550.013.8460627
AGCACAC4139650.012.990529
TATGCCG820800.012.78337445-49
CCGCGCC1489900.012.7724122
GAGCACA4294150.012.5315548
GAAGAGC4345300.012.4206615
ACCGTGC1240800.012.2879571
CGCGATC646350.012.0295653
ACCGCAC1074100.011.8920531
CTCACGC2564550.011.5999683
CTCGTAT895600.011.59625240-44
GCGCGAT647850.011.4546582
CGCGCCC1207600.011.4518073
GCTCACG2628950.011.2112632
CGTATGC962000.010.9462345-49
ACTGCGC1131600.010.6869591
Produced by FastQC (version 0.11.5)