FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400343319
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8095820.20222193342010036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1491750.033.563329
GAGCGTC1795700.027.9825048
ATCGGAA2053900.024.9932881
TCGGAAG2227850.023.0380482
AGAGCGT2222000.022.6972057
CGGAAGA2548700.020.123623
AAGAGCG2773350.018.4655726
ACCGCGC1019450.015.6316211
CGCGATC515050.013.1930443
CTCACGC2890400.011.9024343
GCGCGAT535050.011.8063022
GCTCACG2972700.011.587542
CGCCGTA855500.011.47169255-59
ACCGTGC1179250.011.1380791
GCGATCT1116100.010.4889014
CCTTCGT1388700.010.2629141
ACCGCAC1072300.010.24583151
TCGCCGT962100.010.21570955-59
GAAGAGC5307800.010.0575755
TCACGCC3520500.09.8482724
Produced by FastQC (version 0.11.5)