FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374379690
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1685300.051.6239551
TCGGAAG1780400.048.7444572
CGGAAGA1986250.043.681783
AGAGCAC4085550.021.4845717
AGCACAC4311150.020.4408259
GAGCACA4427100.019.908738
GAAGAGC4512600.019.6212755
CGCGCAT704700.017.89752635-39
TCGCGCA948750.017.62364235-39
TCTCGCG1012400.017.2203430-34
TATGCCG1035200.016.67055345-49
CTCGTAT1130850.015.23794940-44
GCGCATC840150.015.01034235-39
CTCGCGC1144950.014.97506330-34
CGTATGC1177050.014.44362245-49
AAGAGCA6563600.013.7930356
TCTCGTA956350.013.37665340-44
ACTCTCG1327050.013.34573930-34
TCGTATG1287200.013.2531340-44
ATGCCGT1202750.013.20848745-49
Produced by FastQC (version 0.11.5)