FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374130727
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6581440.17591284342705166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1983800.027.6467119
GAGCGTC2267950.024.3357498
ATCGGAA2575100.021.8174611
TCGGAAG2743500.020.417142
AGAGCGT2766200.020.036117
CGGAAGA3071950.018.1822833
AAGAGCG3335250.016.7774936
GAAGAGC5648100.010.2708975
CGCCGTA1101150.09.87255955-59
TCGCCGT1193900.09.10802155-59
ACCGCGC928250.08.7055681
GCCGTAT1316050.08.46638655-59
GTCGCCG1394800.08.09124455-59
CCGTATC1345950.08.06833455-59
TGGCCCG884800.07.8876495
CGTATCA1468700.07.67965460-64
GGATCCG480650.07.2901334
GGTCGCC1564000.07.17891350-54
TGGTCGC1616400.07.09323650-54
TATCGCG254900.06.769913720-24
Produced by FastQC (version 0.11.5)