FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410149363
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG6752060.1646244175685822TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1985900.072.299961
TCGGAAG2094550.068.4321442
CGGAAGA2347650.060.9774443
AGAGCAC4658300.031.1150347
AGCACAC4933650.029.379959
GAGCACA5055050.028.7630928
GAAGAGC5161550.028.1654075
AAGAGCA7359050.020.0536926
TATGCCG1444500.020.04551545-49
CTCGTAT1535850.018.79890840-44
CGTATGC1564250.018.35623245-49
GGAAGAG8412750.017.6399774
TCTCGTA1311800.017.42286140-44
ATGCCGT1632550.016.82931745-49
TGCCGTC1612700.016.49281145-49
TCGTATG1729950.016.40743840-44
GCCGTCT1611700.016.35595550-54
ACTCCGG1872400.015.75426430-34
CACTCCG1880550.015.48422430-34
CCGGAGA1850050.015.36923930-34
Produced by FastQC (version 0.11.5)