FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410149363
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8894010.21684807541685736No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC8420980.2053149598576848Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2272300.060.3358429
GAGCGTC2585600.053.1802838
ATCGGAA2921100.048.2778931
TCGGAAG3076800.045.8002742
AGAGCGT3073100.044.865967
CGGAAGA3440000.040.9110533
AAGAGCG3716150.037.8011936
GAAGAGC6261050.022.8376735
CGCCGTA1457800.018.18353855-59
TCGCCGT1549550.016.97219855-59
GTCGCCG1772200.015.6437655-59
GCCGTAT1690850.015.61901755-59
CCGTATC1731400.014.80540255-59
GGAAGAG10173550.014.3792184
CGTATCA1841900.014.34597860-64
TGGTCGC1991450.013.98385650-54
GGTCGCC1967100.013.90128650-54
GCGTCGT2097050.013.23840210-14
GTGGTCG2147200.013.05254550-54
CGTCGTG2154100.012.91469110-14
Produced by FastQC (version 0.11.5)