FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360673081
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG5698480.15799571135723323TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1644600.065.46451
TCGGAAG1745700.061.6047822
CGGAAGA1901700.056.4675983
AGAGCAC3971050.027.455057
AGCACAC4231550.025.793929
GAGCACA4299800.025.3407888
GAAGAGC4422550.024.761675
TATGCCG1130600.019.24618345-49
CTCGTAT1219500.017.98803340-44
AAGAGCA6342600.017.579176
CGTATGC1245750.017.211345-49
TCTCGTA1138950.016.932840-44
CGCTCAT1305850.016.59535230-34
ATGCCGT1330050.015.88621545-49
TCGTATG1352700.015.82911340-44
ACCGCTC1376000.015.79874930-34
TATCTCG1191100.015.79375840-44
GGATCCG417950.015.7739044
TGCCGTC1319300.015.63676645-49
GGAAGAG7205850.015.5885794
Produced by FastQC (version 0.11.5)