FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441120076
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1344350.033.7763251
TCGGAAG1469050.030.9173872
CGGAAGA1716300.026.4929923
TCGCGCA631600.014.14509335-39
CGCGCAT525300.013.96039435-39
TCTCGCG683600.013.63741430-34
GGATCCG488750.012.32432654
TATGCCG769050.012.32044745-49
TGGCCCG991700.011.3292285
AGAGCAC4215150.011.2739187
GCGCATC679250.011.02897235-39
TATCGCG275600.011.02549420-24
CTCGCGC837800.010.959537530-34
CTCGTAT894150.010.85277340-44
AGCACAC4522100.010.5519499
CGTATGC901000.010.4517745-49
GAGCACA4622800.010.3503158
GAAGAGC4744400.010.2011485
TCTCGTA821900.09.38828340-44
ACTCTCG1048200.09.1884730-34
Produced by FastQC (version 0.11.5)