FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441120076
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1537400.028.2472659
GAGCGTC1872150.023.4017568
ATCGGAA2171900.021.2603321
TCGGAAG2319350.019.8805872
AGAGCGT2295050.019.2128037
CGGAAGA2683950.017.1420783
AAGAGCG2878250.015.9092646
GGATCCG557250.010.7725124
CGCCGTA799300.010.41101155-59
TGGCCCG1090450.09.600595
TATCGCG299100.09.53089320-24
TCGCCGT885300.09.38819755-59
GAAGAGC5729650.08.5106995
GTCGCCG1085450.08.37041755-59
GCCGTAT1062600.08.05370855-59
TGGTCGC1222700.07.52925350-54
CCGTATC1141400.07.36303755-59
CTAGTAC1529850.07.22225149
GATCCGT491600.07.19688275
GGTCGCC1263400.07.140943550-54
Produced by FastQC (version 0.11.5)