FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345500046
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA954600.027.444581
TCGGAAG1053850.024.8219242
CGGAAGA1246350.020.9125523
ACCGCGC894000.013.707531
GGATCCG380200.013.3829644
TGGCCCG741250.011.7241625
TATCGCG197300.010.88912320-24
TATGCCG564150.09.70961645-49
CCGCGCC1267400.09.6764052
CGGCTAT624750.08.78857330-34
CTCGTAT644250.08.72065140-44
AGAGCAC3210000.08.6390757
ATCGCGG255600.08.55854920-24
CGCGCCC1033800.08.46953
ACCGTGC1046150.08.4631431
CGTATGC668850.08.10732545-49
AGCACAC3470500.08.0783379
CCGGGGG1073950.08.0178879
ACTGCGC947650.07.9654951
GAGCACA3559150.07.8934168
Produced by FastQC (version 0.11.5)