FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345500046
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1181200.020.334589
GAGCGTC1429850.017.0265798
ATCGGAA1668650.015.3850441
TCGGAAG1797950.014.2907232
AGAGCGT1724000.014.1887797
CGGAAGA2062250.012.490853
GGATCCG403700.011.9067154
AAGAGCG2161400.011.8876456
ACCGCGC914450.011.1709251
TGGCCCG786750.010.0536835
TATCGCG221000.09.52011820-24
GATCCGT361850.08.25478655
CGCGATC492500.07.58121973
CCGCGCC1403700.07.36001442
CTAGTAC1215050.07.3570919
ACCGTGC1023050.07.34886031
CGCCGTA617150.07.217681455-59
GCGCGAT495550.07.1688042
CCTTCGT1244450.06.85704661
ATCGCGG322500.06.753165220-24
Produced by FastQC (version 0.11.5)