FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358055352
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1378400.042.167341
TCGGAAG1465500.039.72352
CGGAAGA1676400.034.7131083
AGAGCAC3698900.016.2691567
AGCACAC3987150.015.0275739
GAGCACA4040100.014.8700678
GAAGAGC4100700.014.6874165
TATGCCG845200.014.1080945-49
ACCGCGC972350.013.35151
CTCGTAT943600.012.57384740-44
CGTATGC973900.012.31220545-49
ATGCCGT1034550.011.22741945-49
TCTCGTA829600.011.17849340-44
TATCGCG163200.010.9640299
TCGTATG1084800.010.95324540-44
TGCCGTC1031700.010.89865845-49
GCCGTCT1049500.010.58436550-54
AAGAGCA6067450.010.2894866
ACTCCGG1201350.010.14312930-34
CGCGATC572500.09.9331633
Produced by FastQC (version 0.11.5)