FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390365049
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1867600.042.0481451
TCGGAAG1980900.039.4104272
CGGAAGA2157950.036.153133
AGAGCAC4361300.018.3579357
AGCACAC4643350.017.260039
GAGCACA4735100.016.9546728
GAAGAGC4853750.016.5835235
TATGCCG1158900.014.06646245-49
CGTATGC1261350.012.83890145-49
CTCGTAT1096900.012.79986940-44
TCTCGTA1107350.012.29418740-44
ATGCCGT1365200.011.83889145-49
AAGAGCA6992450.011.7652526
TATCTCG1163850.011.53044840-44
GCCGTCT1374150.011.49388350-54
TGCCGTC1348450.011.377460545-49
TCGTATG1208350.010.74709840-44
TGGCCCG833750.010.7018255
TATCGCG221300.010.6448039
ATCTCGT1298550.010.56324140-44
Produced by FastQC (version 0.11.5)