FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390365049
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC4525380.11592687438572401Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2294050.032.6269879
GAGCGTC2618250.028.6314038
ATCGGAA2897700.026.690341
AGAGCGT2863700.026.2882847
TCGGAAG3073150.025.0099832
CGGAAGA3249050.023.5089343
AAGAGCG3386600.022.49856
TCCGATC384800.020.6296863
CCGATCT496800.015.7033584
TTCCGAT564950.014.414582
GAAGAGC5861300.013.447265
CTTCCGA705150.011.7044861
CGCCGTA1278050.011.42373255-59
TATCGCG232850.010.80221320-24
TCGCCGT1405800.010.35983655-59
GCCGTAT1464600.010.05279655-59
GATCCGT397800.09.4426051
GTCGCCG1664700.09.4418455-59
CGTATCA1597050.09.25431260-64
CCGTATC1531250.09.17120555-59
Produced by FastQC (version 0.11.5)