FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410657067
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG8392860.20437636837259154TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2591450.062.8383371
TCGGAAG2701650.059.9367942
CGGAAGA2911800.055.5979083
AGAGCAC5165350.031.7498997
AGCACAC5465850.029.971289
GAGCACA5573150.029.408548
GAAGAGC5709000.028.7582555
AAGAGCA7929400.020.9612396
TATGCCG1787650.019.32142445-49
GGAAGAG8890600.018.7955954
CGTATGC1907050.017.95131945-49
CTCGTAT1663500.017.94081940-44
TCGTATC1927500.017.87758835-39
TCTCGTA1664200.017.64058540-44
ATGCCGT2000100.017.09008445-49
TATCTCG1724750.016.85486240-44
GCCGTCT2010800.016.71447450-54
GTCACGA2124200.016.37261625-29
ATTCGTA2112700.016.31935335-39
TGCCGTC1992900.015.90939345-49
Produced by FastQC (version 0.11.5)