FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005754236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410657067
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC9775190.23803778835248926Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3024850.052.077569
GAGCGTC3367150.046.865398
ATCGGAA3668500.043.9705241
AGAGCGT3628300.043.563377
TCGGAAG3829750.041.9218752
CGGAAGA4043700.039.5763133
AAGAGCG4177550.038.265326
GAAGAGC6786250.023.8667415
TCCGATC433850.023.0596663
CCGATCT530750.018.5239544
CGCCGTA1930350.016.40064655-59
TTCCGAT625700.016.2717572
GGAAGAG10217950.016.1996674
TCGCCGT2053000.015.37282455-59
GCCGTAT2121000.014.968822555-59
GTCGCCG2369100.014.20592355-59
CGTATCA2239200.014.10594460-64
TGGTCGC2418750.013.87655550-54
CTTCCGA750800.013.754681
CCGTATC2199950.013.31532455-59
Produced by FastQC (version 0.11.5)