FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210734
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33130.27363566233375786No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGAA32430.2678540455624439No Hit
GTAATAGACAGCCGTGTCCTCAGGTCTCAGACTCTTCATTTGCAGATACA29860.24662725255919135No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC29550.24406682227475235No Hit
GTCTATTACTGTGCGACTCTTTCCGGGGTGGCAGTTATTTTCCCCTACCAGTACGACGGTTTGGACGTCTGGGGC26840.221683705917237No Hit
GTACACACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT23650.19533605234510637No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20780.17163142358271924No Hit
GGTCTAGTCAGAGCCTCGAATACAGCGACGGAAACATCCACTTGAATTGGTTTCACCAGAGGCCAGGCCAGTCTC20420.1686580206717578No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20400.16849283162114884No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC20090.1659324013367098No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20010.16527164513427392No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19650.1622982422233125No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19330.15965521741356897No Hit
GGTACACACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT18990.15684700355321649No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18390.1518913320349474No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17350.143301501403281No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG16580.13694172295483567No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16560.1367765339042267No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16520.13644615580300876No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16150.1333901583667428No Hit
ACTCTACACTGGGTCCGCCAGGCCCCAGGCAAGGGACTGGAGTGGGTGGC15940.13165567333534864No Hit
AGGTACACACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT15810.13058194450639035No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTCAA15720.12983859377864998No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14740.12174433029881047No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14600.12058800694454769No Hit
GGCCAGGCCAGTCTCCAAGGCGCCTACTTTATAAGGTTTCTAACCGGGAC13940.11513676827445168No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13920.11497157922384274No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13840.11431082302140684No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG13720.11331968871775304No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAAGTAGGCGCCTTGGAGACTGGCCTGGCCTCTGGTGAAA13720.11331968871775304No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGAATA13430.11092444748392297No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12990.10729028837052565No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12990.10729028837052565No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTGAGTGTGAAATCAGTGCCTGACCCACTGCCG12550.10365612925712833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTC200.007380692351.71171614
GTATCAA19900.042.6413461
GGTATCA7600.040.3947871
TATATCG350.00135275939.3994035
TATCAAC22050.038.3049772
GTGGTAT2800.038.190251
TCAACGC23600.035.6431054
CAACGCA23750.035.4179885
ATCAACG24100.035.046673
AACGCAG24300.034.900096
TAGCGTC400.002605829234.4744765
AGAGTAC25650.032.6600311
CAGAGTA26200.032.23758310
ACGGTTT7850.031.59700256
TGGTATC3200.031.2424952
ACGCAGA26950.031.2125137
CGCAGAG27400.030.6999028
GACGGTT8200.030.6551455
AATAGAC8900.030.600943
GTACGAC8000.030.45952251
Produced by FastQC (version 0.11.5)