FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210734
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31600.2609986999621717No Hit
GTAATAGACAGCCGTGTCCTCAGGTCTCAGACTCTTCATTTGCAGATACA29890.2468750361351048No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC29020.23968931243361466No Hit
GTCTATTACTGTGCGACTCTTTCCGGGGTGGCAGTTATTTTCCCCTACCAGTACGACGGTTTGGACGTCTGGGGC26490.21879289753158004No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGAA24550.20276955962250995No Hit
GTACACACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT24050.19863983335728577No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20690.17088807285497887No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC19750.16312418747635732No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19270.15915965026174206No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18850.15569068019895368No Hit
GGTCTAGTCAGAGCCTCGAATACAGCGACGGAAACATCCACTTGAATTGGTTTCACCAGAGGCCAGGCCAGTCTC18820.15544289662304023No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18450.15238689918677428No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18210.15040463057946668No Hit
GGTACACACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT18120.1496612798517263No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18010.14875274007337697No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17250.14247555615023613No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG16620.1372721010560536No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16410.1355376160246594No Hit
AGGTACACACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT15370.12694778539299303No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15200.12554367846281678No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14070.11621049710340998No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13960.11530195732506066No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTCAA13960.11530195732506066No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGAATA13630.11257633799001267No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAAGTAGGCGCCTTGGAGACTGGCCTGGCCTCTGGTGAAA13420.1108418529586185No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTGAGTGTGAAATCAGTGCCTGACCCACTGCCG13250.10943774602844225No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13000.10737288289583012No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG12920.10671212669339425No Hit
GGCCAGGCCAGTCTCCAAGGCGCCTACTTTATAAGGTTTCTAACCGGGAC12450.10283018400408347No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12390.10233461685225657No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12240.1010956989726893No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12180.10060013182086239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21250.041.441921
TGGTATC3850.039.304582
GTGGTAT4050.039.0716441
GGTATCA8350.037.9018331
TATCAAC24800.035.5009162
ATCAACG26600.033.0985953
TCAACGC26750.033.041564
CAACGCA27300.032.375895
AACGCAG27400.032.257736
AGAGTAC28500.030.67728411
CGCAGAG29350.030.2479558
TCTGGAG11250.030.0928670
CAGAGTA29150.029.99322710
AATAGAC7550.029.1530673
ACGCAGA30500.028.9910457
GTGTATA1551.8189894E-1228.851641
GCAGAGT30950.028.6925499
GAATTGT7600.028.19761762
TAATAGA7600.028.0562322
AATTGTC7750.027.66469463
Produced by FastQC (version 0.11.5)