FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594665
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTGTGTCTGTAGATGTGTCCTCGGT18370.30891342184255No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT15190.25543793564443845No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT12480.20986605904164532No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12430.20902524951022847No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12370.2080162780725282No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA12300.20683914472854462No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT11890.19994450657092647No Hit
GTGTATTACTGTGCAACAGCGGGATTTGTTGGGCAGTGGCTGGTACCTCT11770.19792656369552608No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC10360.17421573490957093No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG10330.1737112491907208No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTCAGCTCCTGATCTATAAGGCGT9320.1567268966561005No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8910.14983225849848233No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8820.148318801341932No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8450.14209681080944733No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8350.14041519174661365No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8310.13974254412148016No Hit
GGGTGCGACAGGCTCCTGGAAAAGGGCTTGAGTGGATGGGAGGTTTTGATCCTGAAAATGGTGAAACAATCTACG8260.13890173459006333No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8220.13822908696492983No Hit
CTATTATAGCGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC8160.1372201155272296No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA8080.13587482027696265No Hit
ATCCATGCACTGGGTGCGACAGGCTCCTGGAAAAGGGCTTGAGTGGATGG8050.13537033455811254No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7940.1335205535889955No Hit
GGTCTAGAGGTACCAGCCACTGCCCAACAAATCCCGCTGTTGCACAGTAA7920.13318422977642874No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGA7830.13167077261987842No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTT7600.12780304877536092No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT7480.12578510589996048No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7310.12292635349314321No Hit
AGCTGGTACAGTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGTATCCGGAGACA7300.12275819158685984No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA7210.12124473443030952No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA6980.117377010585792No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTT6950.11687252486694188No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC6920.11636803914809178No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGA6860.11535906771039156No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT6740.11334112483499113No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA6650.11182766767844081No Hit
GTATCCGGAGACACCCTCACTGAATTATCCATGCACTGGGTGCGACAGGC6610.11115502005330732No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA6550.1101460486156071No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC6440.10829626764649004No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCAACAGCGGGATTTGTTGGG6350.10678281048993972No Hit
GAATTATCCATGCACTGGGTGCGACAGGCTCCTGGAAAAGGGCTTGAGTG6350.10678281048993972No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG6120.1029150866454222No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6090.10241060092657212No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT6040.10156979139515525No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC6000.10089714377002179No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA5990.1007289818637384No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCG5990.1007289818637384No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT5980.10056081995745503No Hit
GTCTAGAGGTACCAGCCACTGCCCAACAAATCCCGCTGTTGCACAGTAAT5980.10056081995745503No Hit
CCCCTACAGAAGCCCCCAGAGCGCAGCACCTCACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAG5950.10005633423860492No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT200.007394466551.684081
GTATCAA8050.041.94651
GTATAAG1250.038.590781
GGGTTAT451.0085238E-438.28451
TATCAAC8800.037.9671942
AACGCAG9300.035.9259456
ATCAACG9450.035.3556943
TCAACGC9500.035.169614
CAACGCA9650.034.6229325
CCTTACA400.002615915834.4444662
GTGGTAT1450.033.2679141
ACGCAGA10200.032.767037
AGAGTAC10150.032.6109211
GGTATCA3400.032.4292261
ATAAGAG1450.030.8812433
CGCAGAG11000.030.0859188
CCTTTGT5400.029.97026870
GATTCAC703.8815328E-529.5337581
TATAAGA1700.028.3660282
AGCCTAG500.00758104727.71168741
Produced by FastQC (version 0.11.5)