FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264452
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACACATTTCA103430.8179828099445452No Hit
GTATATAAGTTACCCTCGGACTTTCGGCGGTGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT98370.7779654743715064No Hit
CTTATATACTGTTGGCAGTAATAAGTTGCAGAATCCTCAGGTTGCAGGCTGCTGATGGTGAGAGTGAAATGTGTC90560.7161995868565987No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT75750.5990737489442066No Hit
GTATAGATCAGGGACTTAGGGGCTTCCCCTGGCCTCTGCTGAAACCAGGC55610.4397952630863014No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49250.38949679386801556No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48390.3826954285334675No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46810.37019989687232097No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45350.3586533929322742No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT44930.3553317959084252No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42470.3358767276258806No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG40040.31665891627361104No Hit
GGCCAGGGGAAGCCCCTAAGTCCCTGATCTATACTGCATCCACTTTGCAA39750.3143654326142867No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37850.29933916036354086No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCACCGCCGAAAGTC36690.2901652257262435No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC34850.2756134673360475No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC34400.27205461338192355No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31410.2484080059978552No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31200.24674720748593068No Hit
ATACTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACACATTTCACTCTCA30330.2398667565079576No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30240.2391549857171328No Hit
GTAGGAGACAGAGTCACCATCACCTGTCGGGCGAGTCAGGGCATTAACTACTATTTAGCCTGGTTTCAGCAGAGG29750.23527978918930886No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28130.22246791495446247No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27820.22001626000828817No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27460.21716917684498896No Hit
CATTTCACTCTCACCATCAGCAGCCTGCAACCTGAGGATTCTGCAACTTATTACTGCCAACAGTATATAAGTTAC27370.2164574060541642No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGTCGGGCGAGTCAGG25700.2032501036021929No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25220.19945399271779396No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGTCGGGCGAGTCAGGGCATTAACTA25180.19913765014409404No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT24600.19455068282544533No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT24520.1939179976780455No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24270.19194085659242105No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24240.19170359966214612No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC23990.18972645857652168No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG23230.18371594967622337No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT22850.18071069522607422No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC22800.18031526700894934No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22740.17984075314839948No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22260.17604464226400054No Hit
CCCCTGGCCTCTGCTGAAACCAGGCTAAATAGTAGTTAATGCCCTGACTCGCCCGACAGGTGATGGTGACT22260.17604464226400054No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22160.17525378582975074No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21770.17216944573617662No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21570.17058773286767706No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC20590.16283733981202925No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20110.1590412289276303No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA19810.15666865962488097No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19720.15595688883405617No Hit
CACCATCAGCAGCCTGCAACCTGAGGATTCTGCAACTTATTACTGCCAACAGTATATAAGTTACCCTCGGACTTT19510.15429609032213165No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19330.15287254874048203No Hit
GTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA18080.1429868433123598No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17650.13958616064508578No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17320.1369763344120615No Hit
GTCCCTGATCTATACTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGT17040.13476193639616213No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16940.13397107996191235No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16720.13223119580656284No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16610.1313612537288881No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16510.1305703972946383No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16430.12993771214723848No Hit
CTACTATTTAGCCTGGTTTCAGCAGAGGCCAGGGGAAGCCCCTAAGTCCC16330.12914685571298873No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16280.12875142749586382No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16190.12803965670503903No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15990.12645794383653947No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15960.12622068690626453No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15960.12622068690626453No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15920.12590434433256462No Hit
ACTTATTACTGCCAACAGTATATAAGTTACCCTCGGACTTTCGGCGGTGGGACCAAGGTGGAGATCAAACGAA15710.12424354582064008No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15550.12297817552584045No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC15400.12179189087446578No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACACATTTCACT15330.12123829137049094No Hit
AGTCAGGGCATTAACTACTATTTAGCCTGGTTTCAGCAGAGGCCAGGGGAAGCCCCTAAGTCCCTGATCTATA15230.12044743493624115No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14980.1184702938506167No Hit
GGTTTCAGCAGAGGCCAGGGGAAGCCCCTAAGTCCCTGATCTATACTGCATCCACTTTGCAAAGTGGGGTCCCAT14700.11625589583471732No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14590.11538595375704258No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14460.11435784039251785No Hit
TCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGTCGGGC14460.11435784039251785No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACACATTTCACTCTCACCATCAGCAGCCTGCAACCTGAGGATTCTG14370.11364606960169307No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT14290.11301338445429325No Hit
GTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGAC14270.11285521316744329No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14260.11277612752401832No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14240.11261795623716835No Hit
GTAATAAGTTGCAGAATCCTCAGGTTGCAGGCTGCTGATGGTGAGAGTGAAATGTGTCCCAGATCCACTGCCG14220.11245978495031839No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14150.11190618544634355No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13990.1106408151515439No Hit
CAGTATATAAGTTACCCTCGGACTTTCGGCGGTGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT13900.10992904436071911No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13850.10953361614359422No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13820.10929635921331929No Hit
CCATCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGTCG13630.10779373198824471No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13440.10629110476317012No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13380.10581659090262026No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13310.10526299139864542No Hit
GAGTGAAATGTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA13240.10470939189467059No Hit
GCCTGCAACCTGAGGATTCTGCAACTTATTACTGCCAACAGTATATAAGTTACCCTCGGACTTTCGGCGGTGGGA13200.10439304932097065No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13190.10431396367754568No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12950.10241590823534621No Hit
ATGCAGTATAGATCAGGGACTTAGGGGCTTCCCCTGGCCTCTGCTGAAACCAGGCTAAATAGTAGTTAATGCC12870.10178322308794639No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12820.10138779487082152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9350.046.0390471
GTATCAA26900.043.5621451
GTGGTAT2700.043.0212021
TCGTATG2100.041.186340
TGGTATC2900.040.0272942
CTCGTAT2300.039.05117839
ATCCGTA350.001418996439.01820812
TATCAAC30700.038.144432
TCTCCGA2650.037.36177411
CTTATAC2750.037.2697031
CGTATGC2350.036.860941
ATCAACG32800.035.4940763
CAACGCA33150.035.1193285
TCAACGC33150.035.1193284
CACGAGA2850.034.7508921
AACGCAG33800.034.5449646
TATCTCG2600.034.4213236
GATCAGT3000.034.26286730
TATACAC3250.032.5651973
ATCTCCG3050.032.46187210
Produced by FastQC (version 0.11.5)