FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264452
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACACATTTCA101310.8012166535384498No Hit
GTATATAAGTTACCCTCGGACTTTCGGCGGTGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT94400.7465684739317903No Hit
CTTATATACTGTTGGCAGTAATAAGTTGCAGAATCCTCAGGTTGCAGGCTGCTGATGGTGAGAGTGAAATGTGTC88900.7030713700480524No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT73560.5817539930341366No Hit
GTATAGATCAGGGACTTAGGGGCTTCCCCTGGCCTCTGCTGAAACCAGGC52170.41258980174810905No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45580.3604723627310487No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44450.3515356850240262No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43830.3466323751316776No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42070.3327133018888815No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41900.3313688459506569No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38270.30266075738738996No Hit
GGCCAGGGGAAGCCCCTAAGTCCCTGATCTATACTGCATCCACTTTGCAA36390.2877926564234941No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCACCGCCGAAAGTC35470.2805167772283962No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC35270.2789350643598966No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34010.26897027328834944No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33350.2637506208223009No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC31770.25125508916115435No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31480.24896160550183002No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29430.2327490485997096No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28790.22768756742051105No Hit
ATACTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACACATTTCACTCTCA28640.22650128276913636No Hit
GTAGGAGACAGAGTCACCATCACCTGTCGGGCGAGTCAGGGCATTAACTACTATTTAGCCTGGTTTCAGCAGAGG27900.22064894515568798No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27030.21376849417771493No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25490.20158930509026834No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25460.20135204815999344No Hit
CATTTCACTCTCACCATCAGCAGCCTGCAACCTGAGGATTCTGCAACTTATTACTGCCAACAGTATATAAGTTAC25320.2002448491520437No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25060.19818862242299431No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24290.19209902787927102No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24100.19059640065419645No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24010.18988462986337162No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGTCGGGCGAGTCAGG23500.18585126204869776No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGTCGGGCGAGTCAGGGCATTAACTA23460.18553491947499787No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23410.18513949125787296No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT22820.1804734382957993No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT22420.17731001255880016No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC22410.1772309269153752No Hit
CCCCTGGCCTCTGCTGAAACCAGGCTAAATAGTAGTTAATGCCCTGACTCGCCCGACAGGTGATGGTGACT21930.17343481603097627No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21870.1729603021704264No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21680.1714576749453518No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT21530.17027139029397717No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG21270.16821516356492774No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20560.16260008288175432No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC20250.16014842793558No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20130.15919940021448026No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19660.1554823749735063No Hit
CACCATCAGCAGCCTGCAACCTGAGGATTCTGCAACTTATTACTGCCAACAGTATATAAGTTACCCTCGGACTTT19060.15073723636800765No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA18970.15002546557718285No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18860.14915552349950809No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18670.1476528962744335No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18400.14551758390195912No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC18380.14535941261510915No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACACATTTCACT17570.13895347549768594No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17290.13673907748178657No Hit
GTCCCTGATCTATACTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGT17120.13539462154356197No Hit
GAGTGAAATGTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA17000.1344455938224622No Hit
ACTTATTACTGCCAACAGTATATAAGTTACCCTCGGACTTTCGGCGGTGGGACCAAGGTGGAGATCAAACGAA16920.1338129086750624No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16760.13254753838026276No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15870.12550891611543974No Hit
CTACTATTTAGCCTGGTTTCAGCAGAGGCCAGGGGAAGCCCCTAAGTCCC15850.1253507448285898No Hit
GTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA15830.12519257354173982No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15730.12440171710749004No Hit
AGTCAGGGCATTAACTACTATTTAGCCTGGTTTCAGCAGAGGCCAGGGGAAGCCCCTAAGTCCCTGATCTATA15530.12282000423899048No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15320.12115920572706596No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15120.1195774928585664No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15050.11902389335459157No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14970.11839120820719172No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14830.11728400919924205No Hit
GTAATAAGTTGCAGAATCCTCAGGTTGCAGGCTGCTGATGGTGAGAGTGAAATGTGTCCCAGATCCACTGCCG14780.11688858098211716No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14660.11593955326101742No Hit
GGTTTCAGCAGAGGCCAGGGGAAGCCCCTAAGTCCCTGATCTATACTGCATCCACTTTGCAAAGTGGGGTCCCAT14410.11396241217539298No Hit
GTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGAC14370.11364606960169307No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14330.11332972702799315No Hit
CAGTATATAAGTTACCCTCGGACTTTCGGCGGTGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT14310.11317155574114321No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14200.11230161366346844No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14130.11174801415949359No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14100.11151075722921867No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14030.11095715772524382No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13950.11032447257784399No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13930.11016630129099404No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13720.10850550277906951No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13590.1074773894145448No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13530.10700287555399494No Hit
TCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGTCGGGC13520.10692378991056996No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACACATTTCACTCTCACCATCAGCAGCCTGCAACCTGAGGATTCTG13320.10534207704207041No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13190.10431396367754568No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13180.10423487803412071No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT12900.10202048001822132No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG12810.10130870922739653No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12810.10130870922739653No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12790.10115053794054658No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12780.10107145229712161No Hit
ATGCAGTATAGATCAGGGACTTAGGGGCTTCCCCTGGCCTCTGCTGAAACCAGGCTAAATAGTAGTTAATGCC12700.10043876714972176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)