FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101654
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTACACAGCCGTGTCCTTAGGTCTCAGGCTGTTCATTTGCAGATAGA32540.2953740466607483No Hit
GTGTACTACTGTGCGAGAGGGGGCTTTGGTTTAGGGAGTAATTTTAAGCTCTTCTACGGTATGGACGTCTGGGGC24200.21966969665611888No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAACTTCCAGGAACAGC21700.19697654617511487No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21590.1959780475539507No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCGACCCACTC20980.1904409188365857No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19690.17873125318838765No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC18560.16847394917097383No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18190.16511536289978523No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG18080.16411686427862104No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17930.1627552752497608No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17530.15912437117280015No Hit
CTCCTGTACTGCCTCTGGAGTCATCCTCAGTACTCATATTCTACACTGGG17080.15503960408621945No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC16770.15222565342657496No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15810.1435114836418694No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14520.13180181799367133No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14370.13044022896481108No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13410.12172605918010557No Hit
GTGATGGCCAGGGAGGCAGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATTGGTG13330.12099987836471343No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13260.12036447015124532No Hit
GGTTATGATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTGGCACCAATCGG13260.12036447015124532No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13250.1202736975493213No Hit
GTCCTATGACAGCAGCCTGAGTGGGTCGGTGGTATTCGGCGGAGGGACCA12980.11782283729737286No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12450.11301188939540001No Hit
GATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCAT12290.11155952776461574No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG11640.1056593086395547No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT11280.10239149497029013No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11250.10211917716451807No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11190.10157454155297399No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11100.10075758813565784No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11080.1005760429318098No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11060.10039449772796177No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11050.10030372512603776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15500.043.8012581
GGTATCA5750.038.3587271
TATCAAC18150.037.383962
TCGTATG753.893001E-836.93489540
TCAACGC20150.033.5024644
CAACGCA20300.033.254915
ATCAACG20450.033.179413
TGGTATC2500.033.064882
AACGCAG21050.032.0700576
GTGTATA1359.094947E-1230.6337071
AGAGTAC23000.029.20132311
CTCGTAT953.1100353E-729.15114439
CGCAGAG23600.028.3129738
CAGAGTA23850.027.87177810
TAACCGG500.007759976727.5816129
CGTATGC1155.4133125E-827.11381541
GCAGAGT24850.026.7501779
TATACAC1306.4192136E-926.4942973
TTAACGG700.001260496324.88305550
AGTACGG23950.024.30395513
Produced by FastQC (version 0.11.5)