FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101654
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTACACAGCCGTGTCCTTAGGTCTCAGGCTGTTCATTTGCAGATAGA28840.26178818394886233No Hit
GTGTACTACTGTGCGAGAGGGGGCTTTGGTTTAGGGAGTAATTTTAAGCTCTTCTACGGTATGGACGTCTGGGGC25310.22974545546968467No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAACTTCCAGGAACAGC21750.19743040918473498No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20810.18889778460387743No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18360.1666584971324935No Hit
CTCCTGTACTGCCTCTGGAGTCATCCTCAGTACTCATATTCTACACTGGG18210.16529690810363326No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18160.16484304509401318No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18130.16457072728824113No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17530.15912437117280015No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG17330.15730891913431985No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCGACCCACTC15940.1446915274668816No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15690.14242221241878122No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG15580.14142371379761703No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC14240.12926018513979887No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13640.12381382902435792No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC13320.1209091057627894No Hit
GGTTATGATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTGGCACCAATCGG13020.11818592770506892No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12830.11646124826851262No Hit
GATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCAT12700.11528120444350042No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC12580.11419193322041223No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12570.1141011606184882No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12500.11346575240502009No Hit
GTCCTATGACAGCAGCCTGAGTGGGTCGGTGGTATTCGGCGGAGGGACCA12500.11346575240502009No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12390.1124672537838559No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12250.11119643735691968No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11460.10402540180492242No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11120.10093913333950587No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11060.10039449772796177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAT150.002333145669.1662538
GTATCAA17300.042.530491
TATCAAC19000.038.8884282
GGTATCA6350.037.3601041
ATCAACG20900.034.8598183
TCAACGC21000.034.6938174
CAACGCA21500.034.2066735
AACGCAG21650.033.9696776
TGGTATC2650.032.4212462
AGAGTAC22850.031.4594111
CGCAGAG23850.030.7144138
CAGAGTA23600.030.6053810
GCAGAGT24300.030.1479
TTAGGTC9100.027.97179220
GTATAGA500.00786503927.50571
AGTACAC9550.027.3492723
GTCCTTA9650.026.7291416
CTTAGGT10000.025.44739319
ACGCAGA29200.025.0755587
TCCTTAG10300.025.03211817
Produced by FastQC (version 0.11.5)