FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098459
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC44080.40128944275571504No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT36500.3322836810477223No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC34120.3106169643109119No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33620.3060651330636828No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30990.2821225007032579No Hit
GACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGACCATTCAGA30690.2793914019549205No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30050.2735650579584673No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28600.26036474734150294No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTC27300.24852998609870736No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC25820.23505656560690932No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25390.23114199073429234No Hit
ATAATAATCAGCCTCATCCCCAGACTGGAGTCCACTGATGGCCAGGGAGG25000.22759156236145361No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24360.2217652183650004No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23730.2160299109934918No Hit
GCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGACCATTCAGACTGTCATCCCATG23690.21566576449371347No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23490.21384503199482183No Hit
GATTATTATTGTGCAGCATGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTA22800.20756350487364572No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21990.2001895382531346No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT20760.1889920333849511No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20690.18835477701033904No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20250.18434916551277744No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG20010.1821642865141075No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19600.17843178489137965No Hit
GCCCTGAGGGCCGCTGATTATTCCTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGGTCA19530.17779452851676758No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19280.17551861289315304No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18470.16814464627264195No Hit
CCTCAGGGCTCCCTGACCGGTTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGT18260.16623287714880572No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18220.1658687306490274No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18150.16523147427441534No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17670.16086171627707543No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17490.15922305702807296No Hit
GGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCCCC17380.15822165415368256No Hit
TCCCCAGACTGGAGTCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAACCGGTCAGGGAGC17370.158130617528738No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17300.15749336115412593No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17160.15621884840490177No Hit
GCATGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGG17120.15585470190512346No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16870.15357878628150892No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16700.15203116365745103No Hit
GTCATCCCATGCTGCACAATAATAATCAGCCTCATCCCCAGACTGGAGTC16640.15148494390778355No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15840.14420201391221701No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA15690.14283646453804832No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA15660.14256335466321454No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15400.14019640241465545No Hit
ACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGACCATTCAGAC15280.13910396291532046No Hit
ATCCCATGCTGCACAATAATAATCAGCCTCATCCCCAGACTGGAGTCCAC15230.13864877979059756No Hit
CTCTTGTTCTGGAAGCAGATCCAATATCGGCGGTAATTCTGTGACCTGGTACCAGCAACTCCCAGGAACGGCCCC15190.13828463329081922No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG14840.1350983514177589No Hit
TGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCC14770.13446109504314682No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG14540.13236725266942145No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14540.13236725266942145No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG14270.12990926379591775No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGAC14260.12981822717097316No Hit
GTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC14240.129636153921084No Hit
GTCTGGGGATGAGGCTGATTATTATTGTGCAGCATGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGAC14170.12899889754647192No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC13860.1261767621731899No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13830.12590365229835615No Hit
GTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGGTCACAGAATTACCG13680.12453810292418743No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT13570.12353670004979704No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13540.12326359017496329No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT13420.12217115067562831No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13190.12007730830190294No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGTCTGGGGATG13070.11898486880256796No Hit
CTGTCATCCCATGCTGCACAATAATAATCAGCCTCATCCCCAGACTGGAG12960.11798346592817757No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC12790.11643584330411967No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12680.11543444042972928No Hit
GTTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGAC12530.11406889105556056No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12520.11397785443061598No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12510.1138868178056714No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGATCCAATATCGGCGGTAATTCT12110.11024535280788814No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12090.11006327955799897No Hit
GCCCCCAAACTCCTCATTTATAGGAATAATCAGCGGCCCTCAGGGCTCCCTGACCGGTTCTCTGGCTCCAAGT12070.10988120630810981No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG11930.10860669355888568No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA11920.10851565693394111No Hit
ACACCGGACCATTCAGACTGTCATCCCATGCTGCACAATAATAATCAGCC11820.10760529068449527No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGATCCAATATCGGCGGTAATTCTGTGAC11610.10569352156065907No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT11590.1055114483107699No Hit
GGAATAATCAGCGGCCCTCAGGGCTCCCTGACCGGTTCTCTGGCTCCAAG11580.10542041168582533No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG11580.10542041168582533No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11370.1035086425619891No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC11270.1025982763125433No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11190.10186998331298663No Hit
GATTATTCCTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGG11180.10177894668804206No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC11040.10050443393881793No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG11040.10050443393881793No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCG306.5072934E-445.7154356
GGTATCA9150.041.6162831
GTATCAA22050.041.0730321
TATCAAC24950.036.141762
TCAACGC26600.034.028784
ATCAACG26700.033.6445053
CAACGCA27250.033.2170875
AACGCAG27850.032.6245736
TCGTATG1800.030.79658740
AATCGTT703.0667325E-530.57427666
TAAGGTA450.00476506330.4769575
ACGCAGA30350.029.7099157
CGTATGC1900.029.20123341
CGCAGAG31000.029.1975618
TATACAC2350.029.1800633
TATGCCG2150.029.09038543
AGAGTAC31250.028.63484611
CAGAGTA31900.028.05137610
ACGACTT500.00797431727.4280114
TCTCGTA2150.027.33303338
Produced by FastQC (version 0.11.5)