FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166831
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33120.2838457325868099No Hit
GTCTAGAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA28660.24562254516720933No Hit
GGCCAGGGCAGTCTCCACAGTTACTGATCCAAGTGGGATCTGAGCGGGCC27510.2357667905635006No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25640.21974047655573087No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGGGTCAGGGAGAAGTGGTTATGGGAGGGATCGATTGA24640.21117025516120155No Hit
CCCCTGGAGAGCCGGCCTCCATTTCCTGCAGGTCTGGTCAGAGCCTCCTG24030.20594242011053873No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTCATGCTCTGGGT21630.18537388876366845No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21340.18288852455925494No Hit
GCCCTGGCCTCTGCAAAAACCAATCCAAATAGTTGTGTCCGTTATAATGC20480.17551813415995976No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20380.17466111202050683No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20380.17466111202050683No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20310.17406119652288976No Hit
GTTTCTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATGTCCAATGTAAAAT19840.17003319246746101No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19450.1666908061235946No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA18700.16026314007769762No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG17230.1476649146277396No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16630.142522781791022No Hit
GTCTCCGGTGGCTCCATCTCCTACGGTAGTTTCTACTGGAGTTGGATACG16220.139008991019265No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATGTCCAATGTAAAATCTGTGCCTGATCCACTGCCA16040.13746635116824973No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15960.1367807334566874No Hit
GGCCAAAAGTGTACGGAGTTTCTAGACCTTGCATGCAGTAATAAACCCCA15610.13378115596860216No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACATTGGA15610.13378115596860216No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15390.1318957072618057No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15100.1294103430573922No Hit
GCTCCTGGGGCTGCTCATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTCCACGCTCC14940.1280391076342675No Hit
GTCAGGGACCCCGGAGGCCCGCTCAGATCCCACTTGGATCAGTAACTGTGGAGACTGCCCTGGCCTCTGCAAAAA14910.12778200099243164No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14780.12666787221114284No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14650.125553743429854No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14310.12263986815571407No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13510.11578369104009063No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13420.115012371114583No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13340.11432675340302066No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGTTT13120.11244130469622421No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13020.11158428255677129No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12940.11089866484520895No Hit
CACCTGCACTGTCTCCGGTGGCTCCATCTCCTACGGTAGTTTCTACTGGA12860.11021304713364659No Hit
GCTCATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTCCACGCTCCCTGGCCGTCACCC12740.1091846205663031No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12700.10884181171052193No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12530.10738487407345193No Hit
GTCTATTACTGTGCGAGAGGCGGGGCAGCAGCTGGGACTCACTACTATCA12310.1054994253666555No Hit
GGTCAGAGCCTCCTGCATTATAACGGACACAACTATTTGGATTGGTTTTT12260.10507091429692904No Hit
GTCTCCACAGTTACTGATCCAAGTGGGATCTGAGCGGGCCTCCGGGGTCC12240.10489950986903845No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACATTGGACATCAGCAGAG12180.10438529658536669No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11930.10224274123673437No Hit
GGTCTGGTCAGAGCCTCCTGCATTATAACGGACACAACTATTTGGATTGGTTTTTGCAGAGGCCAGGGCAGTCTC11920.10215703902278907No Hit
GCATTATAACGGACACAACTATTTGGATTGGTTTTTGCAGAGGCCAGGGC11860.10164282573911733No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11760.1007858035996644No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA11730.10052869695782851No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTA350.006259651453.9084970
CGTAAAT200.00702826352.3554253
TCGTATG1050.049.4750440
CTCGTAT1100.047.20173339
CGTATGC1150.045.19236441
GTGGTAT3450.039.8988651
GTATCAA20700.039.400131
TAAGACG400.002635420334.3950774
TATCAAC24000.033.9651372
GGTATCA9150.033.4725341
TTATACA1850.033.4654772
CTTATAC1650.033.369961
GTTACGA450.00314643933.17342868
ATGCGTA450.00314643933.17342868
TGTATAA1350.033.12402314
TATACAC2000.032.675323
ATCAACG25600.032.2453843
TGTTACG450.00383104531.86658367
TGGTATC4650.031.80622
CAACGCA26200.031.506945
Produced by FastQC (version 0.11.5)