FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166831
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32780.28093185731266995No Hit
GTCTAGAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA27040.23173878650807186No Hit
GGCCAGGGCAGTCTCCACAGTTACTGATCCAAGTGGGATCTGAGCGGGCC24050.20611382453842927No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGGGTCAGGGAGAAGTGGTTATGGGAGGGATCGATTGA23900.2048282913292499No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21440.18374554669870788No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTCATGCTCTGGGT21110.1809173736385132No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20890.17903192493171677No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20690.1773178806528109No Hit
CCCCTGGAGAGCCGGCCTCCATTTCCTGCAGGTCTGGTCAGAGCCTCCTG20420.175003920876288No Hit
GTTTCTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATGTCCAATGTAAAAT20180.17294706774160096No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19970.17114732124874982No Hit
GCCCTGGCCTCTGCAAAAACCAATCCAAATAGTTGTGTCCGTTATAATGC19130.1639483352773452No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19100.16369122863550933No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18270.15657794487805005No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATGTCCAATGTAAAATCTGTGCCTGATCCACTGCCA17220.1475792124137943No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA17120.14672219027434136No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG16920.1450081459954355No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16780.14380831500020141No Hit
GGCCAAAAGTGTACGGAGTTTCTAGACCTTGCATGCAGTAATAAACCCCA15890.13618081795907033No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15320.13129579176418865No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14850.12726778770875988No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14800.1268392766390334No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14610.12521093457407284No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14470.12401110357883875No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACATTGGA14400.1234111880812217No Hit
GTCAGGGACCCCGGAGGCCCGCTCAGATCCCACTTGGATCAGTAACTGTGGAGACTGCCCTGGCCTCTGCAAAAA14320.12272557036965936No Hit
GTCTCCGGTGGCTCCATCTCCTACGGTAGTTTCTACTGGAGTTGGATACG14300.12255416594176877No Hit
GCTCCTGGGGCTGCTCATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTCCACGCTCC13900.11912607738395706No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13630.11681211760743415No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13410.1149266689006377No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG12740.1091846205663031No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12670.10858470506868603No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGTTT12500.10712776743161606No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12360.10592793643638197No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA12340.10575653200849137No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12260.10507091429692904No Hit
GGTCTGGTCAGAGCCTCCTGCATTATAACGGACACAACTATTTGGATTGGTTTTTGCAGAGGCCAGGGCAGTCTC12190.10447099879931199No Hit
GGTCAGAGCCTCCTGCATTATAACGGACACAACTATTTGGATTGGTTTTT12180.10438529658536669No Hit
CACCTGCACTGTCTCCGGTGGCTCCATCTCCTACGGTAGTTTCTACTGGA12150.10412818994353082No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12110.10378538108774965No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11900.1019856345948985No Hit
GTCTCCACAGTTACTGATCCAAGTGGGATCTGAGCGGGCCTCCGGGGTCC11670.10001448367415676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)