FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805528
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA27150.33704601205668827No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16320.20260003376667227No Hit
GGTCTATACTTTGCATACAGTAATAAACCCCAACATCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAAT15250.1893168207684897No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12210.1515775987923449No Hit
GCAGAAGACAGGGCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAACCAATTCTCTGGAGTGCCACATCGGTT11350.1409013715227776No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11090.1376736749063968No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10920.13556325788799398No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10550.13096999731852896No Hit
CCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTC9590.11905234827343059No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9530.11830749520811196No Hit
CCACTATCTCTGTCCGTCACCCCTGGGCAGCCGGCCTCCATCTCCTGCAA9400.11669364689992155No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9210.11433494552641249No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9030.11210038633045653No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA8780.10899683189162886No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG8260.10254143865886722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14350.040.156871
TATCAAC15900.035.792612
GTGGTAT3100.034.5060541
ATCAACG16700.034.284533
CAACGCA16850.034.1840175
AACGCAG17250.033.1913956
TCAACGC17200.033.0873574
CACGGAT3450.031.80676770
TGGTATC3550.031.0905342
GGTATCA6500.030.2591551
AGAGTAC18850.029.4573811
ACGCAGA19250.029.3827697
CAGAGTA18900.029.3794510
GCAGAGT20400.027.7263879
CGCAGAG20750.027.2587138
AGTACGG18950.023.11394713
TTAACGG902.095525E-423.0484135
GTATTAG1202.4525762E-623.0040361
GTCCTAC4350.023.0040361
TCAAACG8700.022.7430146
Produced by FastQC (version 0.11.5)