FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826679
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15550.041.9334341
GGTATCA6300.041.0723651
TATCAAC18950.033.6672482
ATCAACG20300.031.4282913
CAACGCA20400.031.105185
TCAACGC21050.030.308524
AACGCAG21350.029.8826396
GTGGTAT2600.029.1929721
AGAGTAC21550.028.96694211
CAGAGTA21550.028.96694210
ACGCAGA22550.028.13957
TTAACGG1254.0708983E-927.64914935
GATATAA756.160489E-527.6006321
TATTCTA2055.2588803E-827.18737270
CGCAGAG23650.026.9765038
TAGTACC809.5847194E-525.8646344
CTAGTGA1200.001019140125.80283270
TTTAACG1359.247742E-925.5995134
GCAGAGT24850.025.5365839
TAACGGC1551.4861143E-924.53490636
Produced by FastQC (version 0.11.5)