FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1426226
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA161741.134041869942071No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT109240.7659375162141203No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC73820.5175897788989964No Hit
GGTCTATACTTTGCATACAGTAATAAACCCCAACATCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAAT71400.5006219210700128No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC65800.46135745667236466No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT62980.441584994243549No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC61310.4298757700392505No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG55730.390751535871594No Hit
GCAGAAGACAGGGCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAACCAATTCTCTGGAGTGCCACATCGGTT55230.387245780121804No Hit
CCACTATCTCTGTCCGTCACCCCTGGGCAGCCGGCCTCCATCTCCTGCAA54350.3810756500021736No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG54350.3810756500021736No Hit
CCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTC53930.37813081517235No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC52930.37111930367276996No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA48780.34202153094951293No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT48360.3390766961196893No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC43470.3047904048867431No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT42120.29532486436231004No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG40900.2867708203328224No Hit
GTCTTCTGCAGGTACCAATACAAATAGGTCTTTCCATCACGATTCAGGAG39900.2797593088332424No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA39450.2766041286584314No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG38940.2730282577936456No Hit
CCCCTGGGCAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTG38420.269382271813864No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG37780.2648949044541328No Hit
ATCTCTGTCCGTCACCCCTGGGCAGCCGGCCTCCATCTCCTGCAAGTCTA36450.2555695941596914No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG36100.2531155651348384No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG35870.251502917489935No Hit
GGATACCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTCCGTCACCCCTGGGCAGCCGG35550.24925923381006937No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGAGATTGTGATGA34420.24133622581554398No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA33830.23719943403079174No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA33620.23572701661587997No Hit
AGTCTAGTCAGAGCCTCCTGAATCGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGACAGGGCAGCCTC32990.23130976437114453No Hit
CTCCTGAATCGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGACAGGGCAGCCTCCACAGCTCCTGAT32330.22668216678142175No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCT31920.22380744706659395No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31630.22177410873171574No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC31630.22177410873171574No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31100.2180580076369383No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31040.2176373169469635No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGA30960.21707639602699713No Hit
TTATTACTGTATGCAAAGTATAGACCTTCCGTACACTTTTGGCCAGGGGA30790.2158844390720685No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30790.2158844390720685No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA30280.21230856820728272No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG30250.2120982228622953No Hit
GTATTGGTACCTGCAGAAGACAGGGCAGCCTCCACAGCTCCTGATCTATG30070.21083615079237092No Hit
GCTAATGCTCTGGATACCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTCCGTCACCC29750.2085924671125053No Hit
GTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTCCGTCACCCCT29710.2083120066525221No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29510.2069097043526061No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA29130.2042453299827657No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT27670.19400852319337888No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA27040.1895912709486435No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA27010.18938092560365608No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAAGGT26670.18699701169379887No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT25560.17921423392926505No Hit
GGCCAAAAGTGTACGGAAGGTCTATACTTTGCATACAGTAATAAACCCCA25560.17921423392926505No Hit
GTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGACAATCAGTGCCG24970.17507744214451287No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT24870.17437629099455487No Hit
GGTCAGGGACAGATTTCACACTGACAATCAGTGCCGTGGAGGCTGAGGAT24830.17409583053457164No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC24560.17220272242968504No Hit
GATTGTGATGACCCAGACTCCACTATCTCTGTCCGTCACCCCTGGGCAGC24460.17150157127972704No Hit
GTTTATTACTGTATGCAAAGTATAGACCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA24450.17143145616473127No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA24280.17023949920980266No Hit
GTGCCGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGCAAAGTATA24040.16855673644990346No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG23950.16792570041494126No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT23640.16575213185007145No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC23490.16470040512513445No Hit
AGACAGGGCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAACCAATTC23460.16449005978014702No Hit
TCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG23370.16385902374518485No Hit
TCTATGAACTTTCCAACCAATTCTCTGGAGTGCCACATCGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACA22680.15902108081047464No Hit
GATTTCACACTGACAATCAGTGCCGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGCAAAGTATAGACCTT22510.15782912385554604No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTC22470.15754866339556284No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC22460.15747854828056704No Hit
CTGACAATCAGTGCCGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTAT22340.15663716690061744No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC21920.15369233207079383No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC21810.15292106580584003No Hit
CCCCTGGGCAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTGAATCGTGATGGAAAGACCTATTTGT21790.15278083557584843No Hit
GACAATCAGTGCCGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGC21510.15081761235596602No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA21370.14983600074602482No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA21050.14759231706615922No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20890.14647047522622642No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC20860.14626012988123901No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT20670.1449279426963188No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20640.1447175973513314No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC20560.144156676431365No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20290.1422635683264784No Hit
GCTCCTGATCTATGAACTTTCCAACCAATTCTCTGGAGTGCCACATCGGT19540.1370049347017934No Hit
GTATGCAAAGTATAGACCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCAC19200.13462102079193622No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT19100.13391986964197822No Hit
ATTCTCTGGAGTGCCACATCGGTTCAGTGGCAGCGGGTCAGGGACAGATT19070.1337095242969908No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA19030.1334290638370076No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18970.1330083731470328No Hit
AGTCAGAGCCTCCTGAATCGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGACAGGGCAGCCTCCACAG18680.13097503481215458No Hit
GTGCCACATCGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGAC18480.1295727325122386No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18110.12697847325739398No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18040.12648766745242337No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG18030.1264175523374276No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17970.12599686164745277No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG17930.1257164011874696No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC17820.12494513492251579No Hit
GATCAGGAGCTGTGGAGGCTGCCCTGTCTTCTGCAGGTACCAATACAAAT17620.12354283262259978No Hit
GTTCATAGATCAGGAGCTGTGGAGGCTGCCCTGTCTTCTGCAGGTACCAA17530.1229117965876376No Hit
ATTCAGGAGGCTCTGACTAGACTTGCAGGAGATGGAGGCCGGCTGCCCAGGGGTGACGGACAGAGATAGTGGAGT17310.12136926405772999No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17280.12115891871274259No Hit
GGCTGAGGATGTTGGGGTTTATTACTGTATGCAAAGTATAGACCTTCCGTACACTTTTGGCCAGGGGACCAAG17130.12010719198780559No Hit
CTCCACAGCTCCTGATCTATGAACTTTCCAACCAATTCTCTGGAGTGCCA17060.11961638618283499No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG16930.11870488968788959No Hit
ATGTGGCACTCCAGAGAATTGGTTGGAAAGTTCATAGATCAGGAGCTGTGGAGGCTGCCCTGTCTTCTGCAGGTA16830.11800373853793159No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16730.11730258738797358No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16480.11554970951307858No Hit
GCCCTGTCTTCTGCAGGTACCAATACAAATAGGTCTTTCCATCACGATTC16460.11540947928308698No Hit
CTGCAGAAGACAGGGCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAA16270.11407729209816676No Hit
GACCCAGACTCCACTATCTCTGTCCGTCACCCCTGGGCAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCT16090.11281522002824237No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA16050.11253475956825916No Hit
CCACAGCTCCTGATCTATGAACTTTCCAACCAATTCTCTGGAGTGCCACA15920.11162326307331376No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15780.11064165146337257No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG15760.11050142123338097No Hit
TCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT15700.11008073054340616No Hit
GGGCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAACCAATTCTCTGG15570.10916923404846075No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15530.10888877358847757No Hit
TCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTG15430.10818762243851957No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15170.10636462944862878No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15080.10573359341366656No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT14870.10426117599875477No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14840.10405083065376736No Hit
GTGTACGGAAGGTCTATACTTTGCATACAGTAATAAACCCCAACATCCTC14790.10370025507878836No Hit
CACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATAC14730.10327956438881356No Hit
GTGGAGGCTGCCCTGTCTTCTGCAGGTACCAATACAAATAGGTCTTTCCA14710.10313933415882195No Hit
GGTTTATTACTGTATGCAAAGTATAGACCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA14450.10131634116893114No Hit
AGCCTCCACAGCTCCTGATCTATGAACTTTCCAACCAATTCTCTGGAGTGCCACATCGGTTCAGTGGCAGCGGGT14450.10131634116893114No Hit
CCACTGAACCGATGTGGCACTCCAGAGAATTGGTTGGAAAGTTCATAGATCAGGAGCTGTGGAGGCTGCC14390.10089565047895635No Hit
GTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGACAGGGCAGCCTCCACAGCTCCTGATCTATGAACTTT14340.10054507490397735No Hit
GCTCTGGATACCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCAC14330.10047495978898155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2350.049.5015531
CTTATAC3300.048.729471
GGTATCA12000.047.9000321
CACGGAT3800.041.99120770
TATACAC3850.040.8579333
GTATCAA31400.040.4251861
AAGGGGG4100.037.99630469
TTATACA4250.037.81712
TATCAAC34050.037.460282
TCGTATG4050.036.73869340
TCACGGG700.00239553435.06958470
CGTATGC4250.035.05333741
ATCAACG36650.034.80283
TGGTATC3250.034.7224122
CGAGACT4250.034.6277623
TCAACGC36950.034.4276854
TGTTAGA18250.033.9646570
ACGAGAC4350.033.82697322
CAACGCA37450.033.69415
AACGCAG37750.033.3357436
Produced by FastQC (version 0.11.5)