FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1426226
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA154121.0806141523152712No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT106050.74357079453046No Hit
GGTCTATACTTTGCATACAGTAATAAACCCCAACATCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAAT104860.7352270958459599No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT63010.4417953395885365No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC62590.43885050475871284No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC61480.431067726994179No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC58670.4113653796803592No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG53630.376027361722476No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA49730.3486824668741139No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC48640.34103991933957173No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48590.3406893437645927No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT46260.3243525219705713No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC45940.3221088382907057No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA45500.3190237732308905No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT43580.30556167115169686No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG43080.30205591540190685No Hit
GCAGAAGACAGGGCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAACCAATTCTCTGGAGTGCCACATCGGTT42110.29525474924731426No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG39870.27954896348825503No Hit
GTCTTCTGCAGGTACCAATACAAATAGGTCTTTCCATCACGATTCAGGAG39680.2782167763033348No Hit
CCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTC39080.2740098694035868No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT38840.27232710664368764No Hit
CCACTATCTCTGTCCGTCACCCCTGGGCAGCCGGCCTCCATCTCCTGCAA38620.27078457411378No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35560.2493293489250652No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA34860.2444212908753592No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA34830.24421094553037176No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG34020.23853162121571198No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32710.22934654115126216No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32410.22724308770138815No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG32260.22619136097645115No Hit
GGCCAAAAGTGTACGGAAGGTCTATACTTTGCATACAGTAATAAACCCCA31970.22415802264157295No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA31490.2207924971217745No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT31190.2186890436719005No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30980.21721662625698873No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC30870.21644535999203493No Hit
TTATTACTGTATGCAAAGTATAGACCTTCCGTACACTTTTGGCCAGGGGA30580.21441202165715673No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG29930.20985453918242972No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29290.20536717182269854No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA28790.20186141607290853No Hit
ATCTCTGTCCGTCACCCCTGGGCAGCCGGCCTCCATCTCCTGCAAGTCTA27570.19330737204342088No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG27470.19260622089346288No Hit
GGATACCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTCCGTCACCCCTGGGCAGCCGG27190.1906429976735805No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAAGGT27100.19001196163861828No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGAGATTGTGATGA27060.18973150117863508No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA26590.1864360907738325No Hit
GTGTGAAATCTGTCCCTGACCCGCTGCCACTGAACCGATGTGGCACTCCA26450.18545447916389127No Hit
GTCCCTGACCCGCTGCCACTGAACCGATGTGGCACTCCAGAGAATTGGTT25820.18103722691915589No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA25450.17844296766431128No Hit
CTCCTGAATCGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGACAGGGCAGCCTCCACAGCTCCTGAT25010.17535790260449605No Hit
GTGCCGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGCAAAGTATA25010.17535790260449605No Hit
GTTTATTACTGTATGCAAAGTATAGACCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA24660.17290387357964304No Hit
GTATTGGTACCTGCAGAAGACAGGGCAGCCTCCACAGCTCCTGATCTATG24210.16974869340483204No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24210.16974869340483204No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCT24030.16848662133490766No Hit
GATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCACTGAACCGATGTG24000.16827627598992026No Hit
AGTCTAGTCAGAGCCTCCTGAATCGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGACAGGGCAGCCTC23890.16750500972496646No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23810.16694408880500003No Hit
CCCCTGGGCAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTGAATCGTGATGGAAAGACCTATTTGT23660.16589236208006303No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23610.16554178650508403No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGA23100.16196591564029825No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22900.16056361334038224No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22620.15860039012049984No Hit
GGTCAGGGACAGATTTCACACTGACAATCAGTGCCGTGGAGGCTGAGGAT22370.15684751224560484No Hit
GATTTCACACTGACAATCAGTGCCGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGCAAAGTATAGACCTT21920.15369233207079383No Hit
CCACGGCACTGATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCACTG21910.15362221695579803No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21730.15236014488587363No Hit
GACAATCAGTGCCGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTATGC21730.15236014488587363No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21680.15200956931089463No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21560.15116818793094502No Hit
TCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG21220.14878427402108782No Hit
GTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGACAATCAGTGCCG21070.14773254729615082No Hit
CTGACAATCAGTGCCGTGGAGGCTGAGGATGTTGGGGTTTATTACTGTAT20990.14717162637618442No Hit
GCTAATGCTCTGGATACCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTCCGTCACCC20840.14611989965124741No Hit
GTGCAGAGATTGTGATGACCCAGACTCCACTATCTCTGTCCGTCACCCCT20750.1454888636162852No Hit
TCCTCAGCCTCCACGGCACTGATTGTCAGTGTGAAATCTGTCCCTGACCCGCTGCCACTGAACCGATGTGGCACT20640.1447175973513314No Hit
CCCCTGGGCAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTG20600.1444371368913482No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20220.14177276252150783No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20120.14107161137154983No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC20120.14107161137154983No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT20030.1404405753365876No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19880.1393888486116506No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA19770.1386175823466968No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19450.13637389866683122No Hit
GTGTACGGAAGGTCTATACTTTGCATACAGTAATAAACCCCAACATCCTC18890.1324474522270664No Hit
GGCTGAGGATGTTGGGGTTTATTACTGTATGCAAAGTATAGACCTTCCGTACACTTTTGGCCAGGGGACCAAG18680.13097503481215458No Hit
GATCAGGAGCTGTGGAGGCTGCCCTGTCTTCTGCAGGTACCAATACAAAT18500.1297129627422302No Hit
GTTCATAGATCAGGAGCTGTGGAGGCTGCCCTGTCTTCTGCAGGTACCAA18450.1293623871672512No Hit
GTATGCAAAGTATAGACCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCAC18290.12824054532731838No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18020.1263474372224318No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18000.1262072069924402No Hit
AGACAGGGCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAACCAATTC17950.1258566314174612No Hit
TCTATGAACTTTCCAACCAATTCTCTGGAGTGCCACATCGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACA17800.12480490469252419No Hit
GATTGTGATGACCCAGACTCCACTATCTCTGTCCGTCACCCCTGGGCAGC17790.12473478957752838No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGAGATTGTGATGACCCAGACTC17660.12382329308258297No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT17510.122771566357646No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17480.1225612210126586No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17340.12157960940271738No Hit
ATTCAGGAGGCTCTGACTAGACTTGCAGGAGATGGAGGCCGGCTGCCCAGGGGTGACGGACAGAGATAGTGGAGT17120.12003707687280978No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17110.11996696175781399No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17020.11933592572285177No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16880.11835431411291059No Hit
GCTCCTGATCTATGAACTTTCCAACCAATTCTCTGGAGTGCCACATCGGT16800.11779339319294417No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC16550.11604051531804917No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16540.11597040020305337No Hit
ATGTGGCACTCCAGAGAATTGGTTGGAAAGTTCATAGATCAGGAGCTGTGGAGGCTGCCCTGTCTTCTGCAGGTA16120.11302556537322976No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16100.11288533514323816No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15830.11099222703835157No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG15770.11057153634837676No Hit
ATTCTCTGGAGTGCCACATCGGTTCAGTGGCAGCGGGTCAGGGACAGATT15740.11036119100338937No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15570.10916923404846075No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15520.10881865847348175No Hit
GTGGAGGCTGCCCTGTCTTCTGCAGGTACCAATACAAATAGGTCTTTCCA15410.10804739220852796No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15400.10797727709353215No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15260.10699566548359096No Hit
GTGCCACATCGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGAC15040.10545313295368336No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15020.10531290272369177No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT15010.10524278760869596No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC14960.10489221203371696No Hit
CTGCAGAAGACAGGGCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAA14880.10433129111375056No Hit
GGTTTATTACTGTATGCAAAGTATAGACCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA14850.10412094576876314No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14520.10180714697390177No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14360.10068530513396895No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA14340.10054507490397735No Hit
GCCCTGTCTTCTGCAGGTACCAATACAAATAGGTCTTTCCATCACGATTC14280.10012438421400255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)