FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983471
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44430.4517672610580281No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33200.33757985746402286No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30700.3121596874742621No Hit
GTTACAATACCCCTCGCGAGCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT30200.30707565347630994No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28940.2942638878014705No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28890.2937554844016753No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28740.29223027420228964No Hit
GTATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT26180.26620002013277466No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25390.25816724641601024No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAGGTGAGCTCGCGAGGGGTATTGTAACT24500.2491176658996554No Hit
ACAATACCCCTCGCGAGCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT24350.24759245570026975No Hit
GAGTTACAATACCCCTCGCGAGCTCACCTTCGGCGGAGGGACCAAGGTGG23880.24281346374219476No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23220.2361025388648979No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23090.23478069002543034No Hit
GGGTATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT22410.2278664037882154No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22280.22654455494874787No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21900.2226806891103042No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACAACTATTTAAATTGGTATCAGCAGAAA21320.21678320967267972No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20530.20875043595591533No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20370.20712354507657063No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20310.2065134609968164No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACAA19720.20051430087923283No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC18360.186685728404803No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18030.18333026596615457No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17520.17814455128824339No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA17460.17753446720848912No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17230.17519581156943112No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17100.17387396272996355No Hit
ACTTACTACTGTCAACAGAGTTACAATACCCCTCGCGAGCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAA16960.17245043321053696No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAATACC16880.1716369877708646No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16880.1716369877708646No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16690.1697050548516428No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16140.16411261745389544No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15960.16228236521463266No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15950.1621806845346736No Hit
GTGGGGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGATTTCACT15930.16197732317475552No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAATACCCCTCGCGAGCT15490.15750337325655764No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15120.15374118809807305No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14920.1517075744988922No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14270.1450983303015544No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAAT14250.1448949689416363No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA14200.1443865655418411No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14060.14296303602241447No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13680.13909917018397086No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13540.13767564066454427No Hit
TTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC13440.13665883386495384No Hit
GTCCCAGATCCACTGCCACTGAATCTTGATGGGACCCCACTTTGCAAACT13060.13279496802651017No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT12970.13187984190687882No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12950.1316764805469607No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC12710.12923614422794366No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAATCTTG12670.1288294215081075No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAATCT12570.12781261470851707No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12440.1264907658690495No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12380.12588068178929526No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAATCTTGATGGGACCCCA12370.12577900110933624No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12290.12496555566966387No Hit
CATCAAGATTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG12150.12354202615023728No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTTTGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATT11950.12150841255105642No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT11740.1193731182719165No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11620.11815295011240799No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11590.11784790807253087No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11550.11744118535269468No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11380.11571261379339096No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11280.11469580699380053No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10980.11164538659502925No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10870.11052689911547978No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT10730.10910336959605316No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10600.10778152075658559No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10540.10717143667683135No Hit
TCTTTGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGATTTCACT10380.10554454579748665No Hit
CCCCTAAGCTCCTGATCTTTGGTGCATCCAGTTTGCAAAGTGGGGTCCCA10310.10483278103777335No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAATACCCCTCGCGAGCTCA10300.10473110035781431No Hit
ACCTTGGTCCCTCCGCCGAAGGTGAGCTCGCGAGGGGTATTGTAACTCTG10240.10412101627806006No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10190.10361261287826484No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10120.10290084811855153No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10050.10218908335883825No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT9900.10066387315945259No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9860.10025715043961642No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA9860.10025715043961642No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAC200.006626041653.14076665
TATCGGT503.4687947E-451.93029870
GTATCAA16700.043.1414681
ATCAACG18350.039.605963
TATCAAC18450.039.3912932
TCAACGC18500.039.2828334
AACGCAG18950.038.3499956
CAACGCA19050.038.1486855
AGAGTAC20150.034.900111
ACGCAGA20800.034.7795567
CGCAGAG20700.034.6268848
GTGGTAT2700.034.307741
TGTTAGA7250.032.23259770
GCAGAGT23450.030.720229
TACCCGT450.004688122330.57823431
CAGAGTA23950.029.9356510
AGTACGG18350.028.41550813
GAGTACG18650.027.95842412
TCGACTC500.00795212827.44339814
TATGTCG500.00797980427.4238645
Produced by FastQC (version 0.11.5)